Fig 1: In vivo implantation of hEMSCs enhanced the expression of pro-angiogenic cytokines after MI. The level of mRNA expression for VEGF (a), angiopoietin 1 (ANG1, b), and angiopoietin 2 (ANG2, c) was measured in the infarcted heart tissue of nude rats at 3 days after cell transplantation by real-time qPCR. Individual values were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Representative Western blot images and quantification of VEGFA, ANG1 and ANG2 (d), Fms-like tyrosine kinase 1 (FLT-1) and TIE2 (e), transforming growth factor ß2 and FGF9 (f) protein levels in the infarcted heart tissue of nude rats at 3 days after cell transplantation. Data are expressed as mean ± SEM. n = 3 or 4/group for all assays
Fig 2: Western blot and quantitative analysis of Ang-1 and Ang-2 expression: cerebral cortex (A) and the hippocampus (B), 6-hour (6h), 1-day (1d), 2-day (2d), and 3-day (3d) post SAH. The ratio of Ang-1/Ang-2 after SAH is exhibited in the cortex (C) and hippocampus (D), respectively. Results are described as mean±standard deviation. Ang-1, angiopoietin-1; Ang-2, angiopoietin-2; SAH, subarachnoid hemorrhage; pSAH, post SAH. *P<0.05. **P<0.01, versus the sham group.
Fig 3: Molecular mechanisms of marizomib sensitized CDDP chemotherapy in HeLa cervical cancer xenografts that are relatively insensitive to CDDP. (A, B) The total tissue lysate was then run on SDS-PAGE and western blotted with antibodies against Ang-1, Tie-2, Flt-3L, SCF, PARP, and caspase 3. GAPDH was used as a loading control. *P<0.05, **P = 0.01, ***P = 0.001, ****P = 0.0001, by ANOVA (Dunnett’s multiple comparison post-test). (C) Localization and expression of Ang-1, Tie-2, Flt-3L, SCF and Ki-67 in cervical tumors.
Fig 4: Cytokine array showing that marizomib boosts the cytotoxic effect of CDDP on cervical cancer cells by upregulating Ang-1 expression and downregulating Flt-3L, SCF and Tie-2 expression. The cervical cancer cell line HeLa was treated with CDDP (40 µM) alone or in combination with marizomib (0.01 µM) for 48 h (A) The cell supernatant was collected to make a cytokine antibody chip, and the PPI map was obtained. (B) The whole cell lysate was then run on SDS-PAGE and western blotted with antibodies against Ang-1, Tie-2, Flt-3L, SCF, PARP, and caspase 3. GAPDH was used as a loading control. (C) The gray value was quantified and plotted. *P<0.05, **P = 0.01, ***P = 0.001, ****P = 0.0001, by ANOVA (Dunnett’s multiple comparison post-test).
Fig 5: Assessment of in vitro anti-metastasis effect and detection of metastasis-related proteins. (A–C) Transwell invasion assay and wound healing rate assay in HCT116 cells after treatment by DHA, CQ, DHA + CQ, LNP/DC, RLNP/DC, and RLNP. (D–F) Expression level of metastasis-related proteins including VEGF, Ang-1, and Paxillin after 24 h of treatment. Each bar represents the mean ± SD (n = 3). & p < 0.05, && p < 0.01, and &&& p < 0.001 vs. Ctrl; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. DHA; ## p < 0.01 and ### p < 0.001 vs. CQ; ^ p < 0.05 and ^^ ^ ^ p < 0.001 vs. DHA + CQ; $$ p < 0.01 vs. LNP/DC.
Supplier Page from Abcam for Anti-Angiopoietin 1 antibody [EPR2888(N)]